SrasV1, Main, Exploration, bibRecord, 002153

An optimal cis-replication stem-loop IV in the 5' untranslated region of the mouse coronavirus genome extends 16 nucleotides into open reading frame 1.

Identifieur interne : 002153 ( Main/Exploration ); précédent : 002152; suivant : 002154

An optimal cis-replication stem-loop IV in the 5' untranslated region of the mouse coronavirus genome extends 16 nucleotides into open reading frame 1.

Auteurs : Bo-Jhih Guan [États-Unis] ; Hung-Yi Wu ; David A. Brian

Source :

Journal of virology [ 1098-5514 ] ; 2011.

RBID : pubmed:21430057

Descripteurs français

KwdFr :
- ARN viral (génétique), Animaux, Cadres ouverts de lecture, Coronavirus bovin (génétique), Lignée cellulaire, Méthode des plages virales, Recombinaison génétique, Régions 5' non traduites, Réplication virale, Viabilité microbienne, Virus de l'hépatite murine (croissance et développement), Virus de l'hépatite murine (génétique), Virus de l'hépatite murine (physiologie).
MESH :
- croissance et développement : Virus de l'hépatite murine.
- génétique : ARN viral, Coronavirus bovin, Virus de l'hépatite murine.
- physiologie : Virus de l'hépatite murine.
- Animaux, Cadres ouverts de lecture, Lignée cellulaire, Méthode des plages virales, Recombinaison génétique, Régions 5' non traduites, Réplication virale, Viabilité microbienne.

English descriptors

KwdEn :
- 5' Untranslated Regions, Animals, Cell Line, Coronavirus, Bovine (genetics), Microbial Viability, Murine hepatitis virus (genetics), Murine hepatitis virus (growth & development), Murine hepatitis virus (physiology), Open Reading Frames, RNA, Viral (genetics), Recombination, Genetic, Viral Plaque Assay, Virus Replication.
MESH :
- chemical , genetics : RNA, Viral.
- chemical : 5' Untranslated Regions.
- genetics : Coronavirus, Bovine, Murine hepatitis virus.
- growth & development : Murine hepatitis virus.
- physiology : Murine hepatitis virus.
- Animals, Cell Line, Microbial Viability, Open Reading Frames, Recombination, Genetic, Viral Plaque Assay, Virus Replication.

Abstract

The 288-nucleotide (nt) 3' untranslated region (UTR) in the genome of the bovine coronavirus (BCoV) and 339-nt 3' UTR in the severe acute respiratory syndrome (SARS) coronavirus (SCoV) can each replace the 301-nt 3' UTR in the mouse hepatitis coronavirus (MHV) for virus replication, thus demonstrating common 3' cis-replication signals. Here, we show that replacing the 209-nt MHV 5' UTR with the ∼63%-sequence-identical 210-nt BCoV 5' UTR by reverse genetics does not yield viable virus, suggesting 5' end signals are more stringent or possibly are not strictly 5' UTR confined. To identify potential smaller, 5'-common signals, each of three stem-loop (SL) signaling domains and one inter-stem-loop domain from the BCoV 5' UTR was tested by replacing its counterpart in the MHV genome. The SLI/II domain (nucleotides 1 to 84) and SLIII domain (nucleotides 85 to 141) each immediately enabled near-wild-type (wt) MHV-like progeny, thus behaving similarly to comparable 5'-proximal regions of the SCoV 5' UTR as shown by others. The inter-stem-loop domain (nt 142 to 173 between SLs III and IV) enabled small plaques only after genetic adaptation. The SLIV domain (nt 174 to 210) required a 16-nt extension into BCoV open reading frame 1 (ORF1) for apparent stabilization of a longer BCoV SLIV (nt 174 to 226) and optimal virus replication. Surprisingly, pleiomorphic SLIV structures, including a terminal loop deletion, were found among debilitated progeny from intra-SLIV chimeras. The results show the inter-stem-loop domain to be a potential novel species-specific cis-replication element and that cis-acting SLIV in the viral genome extends into ORF1 in a manner that stabilizes its lower stem and is thus not 5' UTR confined.

DOI: 10.1128/JVI.00263-11
PubMed: 21430057

Affiliations:

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<term>Recombinaison génétique</term>
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<front><div type="abstract" xml:lang="en">The 288-nucleotide (nt) 3' untranslated region (UTR) in the genome of the bovine coronavirus (BCoV) and 339-nt 3' UTR in the severe acute respiratory syndrome (SARS) coronavirus (SCoV) can each replace the 301-nt 3' UTR in the mouse hepatitis coronavirus (MHV) for virus replication, thus demonstrating common 3' cis-replication signals. Here, we show that replacing the 209-nt MHV 5' UTR with the ∼63%-sequence-identical 210-nt BCoV 5' UTR by reverse genetics does not yield viable virus, suggesting 5' end signals are more stringent or possibly are not strictly 5' UTR confined. To identify potential smaller, 5'-common signals, each of three stem-loop (SL) signaling domains and one inter-stem-loop domain from the BCoV 5' UTR was tested by replacing its counterpart in the MHV genome. The SLI/II domain (nucleotides 1 to 84) and SLIII domain (nucleotides 85 to 141) each immediately enabled near-wild-type (wt) MHV-like progeny, thus behaving similarly to comparable 5'-proximal regions of the SCoV 5' UTR as shown by others. The inter-stem-loop domain (nt 142 to 173 between SLs III and IV) enabled small plaques only after genetic adaptation. The SLIV domain (nt 174 to 210) required a 16-nt extension into BCoV open reading frame 1 (ORF1) for apparent stabilization of a longer BCoV SLIV (nt 174 to 226) and optimal virus replication. Surprisingly, pleiomorphic SLIV structures, including a terminal loop deletion, were found among debilitated progeny from intra-SLIV chimeras. The results show the inter-stem-loop domain to be a potential novel species-specific cis-replication element and that cis-acting SLIV in the viral genome extends into ORF1 in a manner that stabilizes its lower stem and is thus not 5' UTR confined.</div>
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An optimal cis-replication stem-loop IV in the 5' untranslated region of the mouse coronavirus genome extends 16 nucleotides into open reading frame 1.

An optimal cis-replication stem-loop IV in the 5' untranslated region of the mouse coronavirus genome extends 16 nucleotides into open reading frame 1.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

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Pour générer des pages wiki

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